Methods of calculating total hemocyte count in Australian red-claw crayfish

The optimal formula for calculating the total number of hemocytes (HTN) Australian red-claw crayfish (Cherax quadricarinatus) has been established, which allows optimizing time counting HTN without re-ducing accuracy. Methodical recommendations on preparing a hemolymph sample, calculation and differentiated are given. It was found out that differences in minimal when using different formulas, is con-firmed by analysis variance F = 0.011, p 0.998, average values range from 2 384 to 427 cell/µl. Conducting multiple comparisons also demonstrated between formulas minimal: significance levels were 0.99. is: 1 µl N • 10, where 25 large squares Gorjaev’s chamber. chamber should be clean, since even small dust particles grid, hemocyte clusters can form impede counting, or result will incorrect, because distribution 
 grid not uniform. After filling with hemolymph, it recommended wait 1-1.5 minutes so movement stops stop moving. use an anticoagulant, prevents rapid decay cells promotes their uniform developing clots (clusters) affect accuracy counting. Working whole implies speed researcher's actions, quickly coagulates forms hemocytes. In order avoid confusion located border squares, Egorov’s rule applied. increase 400-fold increase.